Flow Cytometer BioCyte-B5R3 BioCyte-B5R3V7
Brand BIOBASE
Product origin CHINA
Delivery time Within 7 days
Supply capacity Factory Direct Supply
Model BioCyte-B5R3 BioCyte-B5R3V7
Introduction:
Flow cytometer is a high-precision analytical and sorting instrument widely used in life sciences, medicine, immunology, and other fields. It integrates fluid mechanics, optics, electronics, and computer technology to rapidly analyze and sort individual cells (or particles) in a liquid suspension. By detecting scattered light signals and fluorescent signals from cells labeled with specific probes, the instrument can quantitatively measure multiple physical and chemical properties of cells (such as size, granularity, surface antigens, and intracellular components) at a speed of thousands to tens of thousands of cells per second, enabling high-throughput, multi-parameter, and non-destructive cellular analysis.
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Features:
Configuration Upgrade
Up to 3 lasers and 15 fluorescent channels are configured, with a maximum of 18 detection parameters for BioCyte-B5R3V7
Powerful Function
High resolution, high sensitivity and high stability
Automatic
Full-automatic detection process, synchronous collection/analysis
Automatic Analysis
Automatic fluorescence compensation adjustment function based on database, without changing the acquisition conditions
Absolute Counting
The absolute counting method based on the volume method of plunger pump is more accurate
Injection Options
The autosampler supports 40-tube Rotary/96-well microplate, which is simple and efficient
High sensitivity and high resolution
Fluorescence sensitivity of Rainbow calibration particles(8 peaks)
Resolution of Rainbow fluorescent particles
BioCyte Design Innovation
New WDM splitter module
The new WDM splitter module not only reduces the number of filters used but also reduces energy loss, further improving the sensitivity of fluorescence detection.
New autosampler with mixing function
Highly efficient and time-saving, compatible with 40-tube rotary/96-well microplate autosampler
Software Features
Chinese and English software, easy to learn and operate, can be switched freely
Modular design, fully automatic detection, simultaneous acquisition and analysis
One-click import of templates, custom editing reports based on needs
Automatic fluorescence compensation adjustment function, no need to change acquisition conditions
BioCyte Flow Cytometer Main applications
Oncology and Hematology
• Analysis of 6-color human peripheral blood immune cell subsets
• Analysis of 9-color human peripheral blood immune cell subsets
• Detection of dendritic cells (DC)
• Phenotypic characterization of natural killer cell(NK) populations
• Characterization of PBMC T cell receptors
• Detection of CD62P Platelet Activation
Immunology
• Detection of T cell proliferation(CFSE)
• Detection of B-cell antibody class
• NK cell-mediated clearance of spleen cells
• Detection of Treg cell subsets
Stem Cells
• Flow cytometry analysis of hematopoietic stem cells from mouse bone marrow
• Detection of CAR-T extracellular tumor killing
• Analysis of mesenchymal stem cells from bone marrow and endometrium of mice
• Flow cytometry analysis of mouse hematopoietic progenitor cells
Cell Biology
• Cell activity test
• Cell cycle detection
• Cell proliferation detection
• Cell apoptosis detection
Oceanography
• Detection of quantity, size and morphology of marine microorganisms
• Detection of DNA content in marine microorganisms
• Determination of Liposome Content in Marine Microalgae
• Detection of marine bacterial activity and membrane integrity
Environmental Monitoring
• Flow cytometric detection of bacterial life and death
• Absolute count of virus, bacteria and algae
• Detection of bacterial periodic ploidy
• Algae classification and counting
Clinical
• Lymphocyte subgroup analysis
• HLA-B27 detection
• Monocyte HLA-DR detection
• Detection of neutrophil CD64
• Regulatory T cell detection
• T cell activation and diseases
• Th1 Th2 detection
• Th17 cell detection
• Blocking Antibody Detection
• Cytokine detection
• Platelet-leukocyte adhesion
• Platelet autoantibody detection
• Platelet activation and diseases
• Paroxysmal nocturnal hemoglobinuria
• Leukemia immunotyping test
• Detection of residual focus of leukemia
• MDS immune typing
• Hematopoietic stem cell count
• Lymphoma immunotyping
• Detection of cell cycle and ploidy
Flow Cytometry Laser/Channel/Fluorochrome Configuration Table
Laser Wavelength | Channel | Filter | Recommended Fluorescent Dyes | BioCyte-B5R3V7 | BioCyte-B5R3 |
488nm | BL1 | 525/40 | FITC, EGFP、BB515, Alexa Fluor 488 | ✓ | ✓ |
BL2 | 575/30 | PE, DsRed | ✓ | ✓ | |
BL3 | 620/30 | PE-Texas Red, ECD, PE-CF594, PI, 7-AAD | ✓ | ✓ | |
BL4 | 697/60 | PerCP, PE-Cy5, PerCP-Cy5.5, PE-Cy5.5, BB700, PerCP-eFluor710 | ✓ | ✓ | |
BL5 | 780/60 | PE-Cy7, PE-Alexa Fluor 750 | ✓ | ✓ | |
638nm | RL1 | 670/20 | APC, Cy5, Alexa Fluor 647 | ✓ | ✓ |
RL2 | 720/40 | APC-R700, Alexa Fluor 680, Alexa Fluor 700 | ✓ | ✓ | |
RL3 | 780/60 | APC-Cy7, APC-eFluor780, APC-H7 | ✓ | ✓ | |
405nm | VL1 | 425/20 | BV421 | ✓ | |
VL2 | 460/40 | Pacific Blue, V450, BV480 | ✓ | ||
VL3 | 525/40 | BV51, V500 | ✓ | ||
VL4 | 605/40 | BV605 | ✓ | ||
VL5 | 660/40 | BV650 | ✓ | ||
VL6 | 720/40 | BV711 | ✓ | ||
VL7 | 780/60 | BV786, BV750 | ✓ |
Technical Parameters:
Model | BioCyte-B5R3 | BioCyte-B5R3V7 |
Software | English/Chinese | |
PC | CPU i5/16G DDR4/1T hard disk+27 inches monitor | |
Power Supply | AC220/110V±10%, 50/60Hz | |
External Size(W*D*H) | 530*450*470mm | |
Net Weight | 35kg | |
Packing Size(W*D*H) | Main Instrument: 860*575*720mm Accessories: 860*680*810mm Reagent: 380*280*320mm | |
Gross Weight | Main Instrument: 67kg Accessories: 53.5kg Reagent: 16kg | |
Recommended Reagent Information:
Item | Recommended Reagent | Application Department |
Lymphocyte Subset T Analysis | CD45 CD4 CD3 CD8 | Clinical Laboratory Infectious Disease Department Oncology Department Department of Rheumatology and Immunology Department of Pediatrics Blood Specialty |
Lymphocyte Subset T/B/NK Analysis | CD45 CD3 CD16 CD56 CD19 | |
Absolute Count of CD4+T Lymphocyte | CD45 CD4 CD3 CD8 | CDC (AIDS) Infectious Disease Department |
Cytokine Th1/Th2/Th17 Analysis | Th1: IL-2, IFN-γ, TNF-α Th2: IL-4, IL-6, IL-10 Th17: IL-17 | Infectious Disease Department Oncology Department Department of Rheumatology and Immunology Department of Organ Transplantation Respiratory Department |
HLA-B27 Detection for AS | HLA-B27-FITC HLA-B7-PE IgG2a-FITC IgG1-PE | Department of Rheumatology and Immunology Orthopedics |
DFI Analysis | Acridine orange (AO) Staining Solution | Reproductive Department Infertility Department Reproductive Medicine Centre |
